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-butyl protecting groups had been removed from the phosphate substituent with trifluroacetic acid. In a 25 mL flask was included the whole quantity of N
b would be the dissociation frequent on the team that must be protonated for whole exercise. The pH profiles which showed which the deprotonation of two acid ic groups as well as protonation of 1 standard team are needed for maximum action ended up fit to equation five the place K
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-acetyl-d-glucosamine-6-phosphate as illustrated in Scheme one. The deacetylation of the compound gives a supply of carbon and nitrogen by making ready this substrate for entry to the glycolytic pathway. This reaction is really a critical stage in the catabolism of N
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